MEKK3 polyclonal, anti-human, mouse, rat

Background:
MAP kinase kinase kinase (MEKK3 or MAP3K3) is a serine/threonine protein kinase that activates SAPK and ERK via phosphorylation and activation of their respective MAP kinase kinases, SEK and MEK1/2. MEKK3 also stimulates MEK5 via activation of ERK5/BMK1, which is at least partly regulated by a direct interaction between MEK5 and MEKK3 via p67phox-Bem1p (PB1) protein-protein interaction domains found in both proteins. MEKK3 modulates NF-κB activation in response to a variety of agonists including TNFα, LPS, IL-1 and LPA. Despite reports showing that phosphorylation of MEKK3 at Ser526 within the activation loop is necessary for kinase activation, at least one study suggests that dual phosphorylation at Thr516 and Ser520 is required for LPA-stimulated IKKβ/NF-κB activation. Phosphorylation at Thr294 appears to negatively regulate MEKK3 by promoting 14-3-3β binding and inhibition of the kinase activity.

Alternative Name:
MEK kinase 3, MEKK 3, MAP3K3, M3K3, MAP/ERK kinase kinase 3, MAP3K3, MAPK/ERK kinase kinase 3, MAPKKK3, MEK kinase 3, MEKK 3, MEKK3, Mitogen activated protein kinase kinase kinase 3, Mitogen-activated protein kinase kinase kinase 3,

Application Dilution:
WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Specificity: MEKK3 polyclonal antibody detects endogenous levels of MEKK3 protein.

Immunogen:
recombinant protein

MW: 71 kDa

Swiss-Prot: Q99759(Human) Q61084(Mouse) Unigene:72680(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.