MEKK3 polyclonal, anti-mouse, rat

Quantity: 100 µg

Background:
MAP3K3(Mitogen-activated protein kinase kinase kinase 3), also known as MAP/ERK KINASE KINASE 3, MEKK3 or MAPKKK3, is an enzyme that in humans is encoded by the MAP3K3 gene. MAP3K3 is contiguously distal to LYK5 on chromosome 17(Puffenberger et al., 2007). By yeast 2-hybrid analysis of a mouse T-cell cDNA library, Uhlik et al.(2003) showed that a C-terminal fragment of mouse Osm(CCM2) interacted with Mekk3, which activates p38 in response to sorbitol-induced hyperosmotic conditions. Mekk3 and Osm colocalized in the cytoplasmic compartment of cotransfected cells, and the Mekk3-Osm complex was recruited to Rac1 and cytoskeletal actin-containing membrane ruffles in response to sorbitol treatment. Protein interaction assays showed that Osm interacted directly with the Mekk3 substrate Mkk3(MAP2K3), with actin, and with both GDP- and GTP-loaded Rac1. Uhlik et al.(2003) concluded that the RAC1-OSM-MEKK3-MKK3 complex is required for regulation of p38 activity in response to osmotic shock.

Description:
Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase kinase kinase 3 (MAP3K3) detection. Tested with WB, IHC-P in Mouse, Rat.

Synonyms:
M3K3_HUMAN antibody, MAP/ERK kinase kinase 3 antibody, MAP3K3 antibody, MAPK/ERK kinase kinase 3 antibody, MAPKKK3 antibody, MEK kinase 3 antibody, MEKK 3 antibody, MEKK3 antibody, mitogen activated protein kinase kinase kinase 3 antibody, Mitogen-activated protein kinase kinase kinase 3 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of mouse MEKK3(11-25aa MKDLVALQMSRRTRL), different from the related rat sequence by two amino acids.A synthetic peptide corresponding to a sequence at the N-terminus of mouse MEKK3(11-25aa MKDLVALQMSRRTRL), different from the related rat sequence by two amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: mouse, rat

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.