Melan A (MART-1) polyclonal, anti-human

Quantity: 200 µl

Background:
Protein melan-A also known as melanoma antigen recognized by T-cells 1 or MART-1 is a protein that in humans is encoded by the MLANA gene.[1] A fragment of the protein, usually consisting of the nine amino acids 27 to 35, is bound by MHC class I complexes which present it to T cells of the immune system. These complexes can be found on the surface of melanoma cells.

Description:
MART-1/MELAN A is a melanocyte differentiation marker recognized by autologous cytotoxi T lymphocytes like seven other melanoma associated antigens (MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, GAGE-1). Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. Histologically, the antigen has been localized in the cytoplasm.

Synonyms:
MART1, MART-1

Isotype: Mouse IgG2b kappa

Immunogen:
Recombinant human MART-1 protein

Form: Ascites containing 0.09% sodium azide

Specificity:
The antibody recognizes a 18 kD protein (corresponding to MART-1, melanoma antigen recognized by T cells 1).

Reactivity: human

Applications: Immunofluorescence, Immunocytochemistry, Immunohistochemistry on frozen and paraffin sections after microwave pretreatment

Working DIlution: 1:10, optimal dilution should be determined by serial dilution

Positive Control:
Melanomas

References:
- Kawakami, Y. et al.(1997) J. Immunol. Methods 202: 13-25