MGMT polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
Methylated-DNA-protein-cysteine methytransferase(MGMT) is an enzyme that in humans is encoded by the MGMT gene. O(6)-alkylguanine is the major mutagenic and carcinogenic lesion in DNA induced by simple alkylating mutagens. MGMT gene is mapped to chromosome 10. Rydberg et al.(1990) concluded that the methyltransferase gene spans at least 15 kb. Gardner et al.(1991) refined the localization of the gene to 10q24.33-qter. Methylation of the gene's promoter plays a significant role in carcinogenesis.

Description:
Rabbit IgG polyclonal antibody for Methylated-DNA--protein-cysteine methyltransferase (MGMT) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
6 O methylguanine DNA methyltransferase antibody, Agat antibody, AGT antibody, AI267024 antibody, EC 2.1.1.63 antibody, Methylated DNA protein cysteine methyltransferase antibody, Methylguanine DNA methyltransferase antibody, MGC107020 antibody, MGMT antibody, MGMT: O-6 methylguanine DNA methyltransferase antibody, O 6 methylguanine DNA alkyltransferase antibody, O 6 methylguanine DNA methyltransferase antibody, O-6 methylguanine DNA alkyltransferase antibody, O-6-methylguanine-DNA methyltransferase antibody, O6 methylguanine DNA methyltransferase antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of human MGMT(87-101aa PVFQQESFTRQVLWK), different from the related rat and mouse sequences by one amino acid.A synthetic peptide corresponding to a sequence in the middle region of human MGMT(87-101aa PVFQQESFTRQVLWK), different from the related rat and mouse sequences by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.