MHC Class II polyclonal, anti-human

Background:
Major histocompatibility complex (MHC) molecules, also designated human leukocyte antigen (HLA) molecules, are cell-surface receptors that bind foreign peptides and present them to T lymphocytes. MHC class I molecules consist of two polypeptide chains, an α or heavy chain and β-2-Microglobulin, a non-covalently associated protein. Cytotoxic T lymphocytes bind antigenic peptides presented by MHC class I molecules. Antigens that bind to MHC class I molecules are typically eight to ten residues in length and are stabilized in a peptide binding groove. MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an α and β chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumu-lating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.

Alternative Name:
HLA-DPB1, D6S221E, DMA, DMB, DP beta 1, DP beta 1 chain, DP(W4) beta chain, DPB 1, DPB1, DPB1, DRB, H2Ea, HLA class II histocompatibility antigen, HLA class II histocompatibility antigen DM beta chain, HLA class II histocompatibility antigen, DP beta 1 chain, HLA class II histocompatibility antigen, DP(W4) beta chain, HLA DMB, HLA DP1A, HLA DPB1, HLA SB alpha chain, HLA-A, HLA-A histocompatibility type, HLA-DP, HLA-DP histocompatibility type, beta-1 subunit, HLA-DP1B, HLA-DPB, HLA-DPB1, HLADM, HLADP1B, HLASB, HLASB histocompatibility type, Human MHC class II HLA SB alpha, LA class II histocompatibility antigen DP alpha 1 chain, Major histocompatibility complex class II, Major histocompatibility complex class II DP alpha 1, Major histocompatibility complex class II DP beta 1, Major histocompatibility complex, class I, A, MHC class II antigen DMB, MHC class II antigen DPB1, MHC class II DP3 alpha, MHC class II DPA1, MHC class II HLA-DP-beta-1, MHC DPB1, MHC HLA DPB1, PLT1, Primed lymphocyte test 1, RING6, RING7,

Application Dilution:
WB:1:500-1:1,000
IP:1:10-1:50
ICC:1:50-1:100
IHC:1:50-1:200

Specificity: MHC Class II polyclonal antibody detects endogenous levels of MHC Class II protein.

Immunogen:
recombinant protein

MW: 29 kDa

Swiss-Prot: P04440(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.