MHC I A,B,C (clone EBS-O-104), anti-human, mouse, monkey

Source:
A CBA mouse was immunized with human PBL from a T-ALL patient.
Fusion partner: NS-1.

Specifications:
EBS-O-104 reacts with a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C). Human MHC class I antigens are expressed constitutively on all nucleated cells lymphocytes such as lymphocytes, thymocytes, granulocytes, and bone marrow cells and also platelets but are absent on erythrocytes. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.

Species reactivity:
Positive: human, monkey, mouse.

Applications:
Demonstration of MHC I A, B, C.
Flow cytometry: +
Frozen sections: +

Positive control: THP-1 and Jurkat cells. Tonsil or lymph node.

References:
- Young NT et al. Hum Immunol52(1): 1-11 (1997).
- Krensky AM et al, Transplant Proc28(6): 3026-8 (1996).
- Hansen JA et al, Hematol Oncol Clin North Am4(3): 507-515 (1990).
- Sparrow RL. Human cell surface antigens defined by monoclonal antibodies. PhD thesis,University of Melbourne, (1983)