MHC II DR (clone LN-3), anti-human, monkey

Source:
A BALB/c mouse was immunized with nuclei from pokeweed mitogen-stimulated PBL.
Fusion partner: NS-1.

Specifications:
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an αand βchain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.

Species reactivity:
Positive: human, monkey. Negative: mouse.

Applications:
Demonstration of MHC II DR.
Flow cytometry: +
Frozen sections: +
Paraffin sections: Citrate

Positive control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node.

References:
- Marder, R.L. et al. Lab. Invest.52: 497-504 (1985).
- Andrade, R.E. et al. Human Pathology19: 932-941 (1988).
- Azumi N. et al. Human Pathology19: 1376-1382 (1988).