MIF (clone 4E4), anti-human

Decription:
The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.

Synonyms: Macrophage migration inhibitory factor, GLIF, MMIF, MIF, EC 5.3.2.1, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, Macrophage migration inhibitory factor, macrophage migration inhibitory factor (glycosylation-inhibiting factor),

Clone: 4E4. Anti-human MIF mAb, clone 4E4, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MIF protein.

Isotype: Mouse IgG1, k

Immunogen: Recombinant human MIF (1-114 aa) purified from E. coli

Reactivity: human

Form: Purified, in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Applications: ELISA, WB (Cell lysate)

Working Dilution: Optimal dilution should be performed by user.

NCBI Nr.: NP_002406

Concentration: 0.1 mg/ml

Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.

References:
Weiser WY, et al., (1989) Proc Natl Acad Sci. 86:7522-7526.
Bernhagen J, et al., (1994) Biochemistry. 33:14144-14155
Richard B, et al., (1996) FASEB J. 10:1607-1613 "