MIF monoclonal, anti-human, mouse, rat

Description: Anti-MIF Rabbit Monoclonal Antibody . Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Protein Function:
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti- inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity..

Other Names: Macrophage migration inhibitory factor, MIF, 5.3.2.1, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, 5.3.3.12, Phenylpyruvate tautomerase, MIF {ECO:0000303|PubMed:2552447, ECO:0000312|HGNC:HGNC:7097}

Clone Name(s):
Clone: ABAG-13

Subcellular Localization: Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non- classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.

Gene ID: 4282

Immunogen: A synthesized peptide derived from human MIF

Calculated MW: 12476

Purification: Affinity-chromatography

Format: Liquid

Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Concentration (mg/ml): Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.