MIG monoclonal, anti-human

Source:
Produced in BALB/c mice using highly pure (>98%) recombinant human MIG as the immunizing antigen. This IgG1k antibody was purified from cell culture by Protein G affinity chromatography.

Western Blot:
To detect hMIG by Western Blot analysis this antibody can be used at a concentration of 0.50-2.0 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIG is 0.25-0.50 ng/lane, under non-reducing conditions and 2.0-4.0 ng/lane, under reducing conditions.

Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000 pg/ml of recombinant human MIG when used with BioGems biotinylated antigen affinity purified anti-human MIG (60-223BT) as the detection antibody at a concentration of approximately 1.0-2.0 μg/ml.

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human MIG (100 ng/ml), a concentration of 5.0-10.0 μg/ml of this antibody is required.

Formulation: A sterile filtered antibody solution was lyophilized from PBS.

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.