Mindin (W104) polyclonal, anti-human, mouse, rat

Background:
The Thrombospondin proteins, Thrombospondins 1-4 and Thrombospondin 5 (also designated COMP), compose a family of glycoproteins that are involved in cell-to-cell and cell-to-matrix signaling. These extracellular, cell-surface proteins form complexes of both homo- and heteromultimers. Spondin-2, or Mindin, is also designated DIL-1 for its differential expression in cancerous and non-cancerous lung cells. Full-length SPON2 cDNA encodes a 331 amino acid protein with a domain arrangement similar to zebrafish F-Spondin and Mindin-1/Mindin-2: an FS1 domain, an FS2 domain, a hydrophobic signal sequence in the N-terminus and a Thrombospondin type I repeat. Immunoblot analysis demonstrates expression of a 42 kDa protein under reducing conditions and expression of dimers and oligomers in a concentration-dependent manner under nonreducing conditions.

Alternative Name:
DIL 1, DIL-1, DIL1, M spondin, Mindin, SPON 2, Spon2, Spondin 2, Spondin-2,

Application Dilution:
WB: 1:500~1:1000

Specificity: Mindin (W104) polyclonal antibody detects endogenous levels of Mindin protein.

Immunogen:
Synthetic peptide, corresponding to amino acids 70-118 of Human Mindin.

MW: ~ 35 kDa

Swiss-Prot: Q9BUD6

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.