MIP-1-beta human active recombinant protein
€295.00
In stock
SKU
600155
Protein Family: Cytokines
Pathway and Disease: Immune System, Signal Transduction, Signaling Molecules and Interaction
Alternate Names: C-C motif chemokine 4, small-inducible cytokine A4, macrophage inflammatory protein 1-beta, MIP-1-beta, MIP-1-beta(1-69), T-cell activation protein 2, ACT-2, PAT 744, Protein H400, SIS-gamma, Lymphocyte activation gene 1 protein, Lag-1, HC21, G-26 T-lmphocyte-secreted protein, MIP-1-beta(3-69), CCL4, LAG1, MIP1B, SCYA4Description:
Macrophage inflammatory protein 1-beta (MIP-1-beta or C-C motif chemokine 4) is a monokine with inflammatory and chemokinetic properties. MIP-1-beta binds to CCR5, and is one of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The N-terminal processed form, MIP-1-beta (3-69) retans the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and inhibits CCR5-mediated HIV-1 entry in T-cells. MIP-1-beta (3-69) is also a ligand for CCR1 and CCR2 isoform B. Recombinant Human MIP-1 produced in E. coli is a single non-glycosylated polypeptide chain containing 69 amino acids with a MW of 7,620 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Human MIP-1 is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract human blood monocytes using a concentration of 2.5 - 15 ng/ml.
Accession No.: P13236
MW: 7620 Da
Sequence:
APMGSDPPTA CCFSYTARKL PHNFVVDYYE TSSLCSQPAV VFQTKRGKQV CADPSESWVQ EYVYDLELN
Purity:
Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug of lyophilized protein. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Immune System, Signal Transduction, Signaling Molecules and Interaction
Alternate Names: C-C motif chemokine 4, small-inducible cytokine A4, macrophage inflammatory protein 1-beta, MIP-1-beta, MIP-1-beta(1-69), T-cell activation protein 2, ACT-2, PAT 744, Protein H400, SIS-gamma, Lymphocyte activation gene 1 protein, Lag-1, HC21, G-26 T-lmphocyte-secreted protein, MIP-1-beta(3-69), CCL4, LAG1, MIP1B, SCYA4Description:
Macrophage inflammatory protein 1-beta (MIP-1-beta or C-C motif chemokine 4) is a monokine with inflammatory and chemokinetic properties. MIP-1-beta binds to CCR5, and is one of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The N-terminal processed form, MIP-1-beta (3-69) retans the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and inhibits CCR5-mediated HIV-1 entry in T-cells. MIP-1-beta (3-69) is also a ligand for CCR1 and CCR2 isoform B. Recombinant Human MIP-1 produced in E. coli is a single non-glycosylated polypeptide chain containing 69 amino acids with a MW of 7,620 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Human MIP-1 is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract human blood monocytes using a concentration of 2.5 - 15 ng/ml.
Accession No.: P13236
MW: 7620 Da
Sequence:
APMGSDPPTA CCFSYTARKL PHNFVVDYYE TSSLCSQPAV VFQTKRGKQV CADPSESWVQ EYVYDLELN
Purity:
Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug of lyophilized protein. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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