MIP-1α monoclonal, anti-human
€0.00
In stock
SKU
AC-ABG10404
Catalog Number: AC-ABG10404
Size(s): 500 µg
Host: Mouse
Applications: WB, E
Datasheet
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Size(s): 500 µg
Host: Mouse
Applications: WB, E
Datasheet
Request Information
Source:
Produced in BALB/c mice using highly pure recombinant human MIP-1alpha as the immunizing antigen. This IgG1k antibody was purified from cell culture by Protein G affinity chromatography.
Western Blot:
To detect Human MIP-1alpha by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-1alpha is 1.0-2.0 ng/lane, under non-reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 2000 pg/ml of recombinant human MIP-1alpha when used with BioGems biotinylated antigen affinity purified anti-human MIP-1alpha (60-224BT) as the detection antibody at a concentration of approximately 1.0-2.0 μg/ml.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human MIP-1alpha (100 ng/ml), a concentration of 5.0-10.0 μg/ml of this antibody is required.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Produced in BALB/c mice using highly pure recombinant human MIP-1alpha as the immunizing antigen. This IgG1k antibody was purified from cell culture by Protein G affinity chromatography.
Western Blot:
To detect Human MIP-1alpha by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-1alpha is 1.0-2.0 ng/lane, under non-reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 2000 pg/ml of recombinant human MIP-1alpha when used with BioGems biotinylated antigen affinity purified anti-human MIP-1alpha (60-224BT) as the detection antibody at a concentration of approximately 1.0-2.0 μg/ml.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human MIP-1alpha (100 ng/ml), a concentration of 5.0-10.0 μg/ml of this antibody is required.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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