MIP-3β monoclonal, anti-human
€0.00
In stock
SKU
AC-ABG10420
Catalog Number: AC-ABG10420
Size(s): 500 µg
Host: Mouse
Applications: WB, IHC, E
Datasheet
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Size(s): 500 µg
Host: Mouse
Applications: WB, IHC, E
Datasheet
Request Information
Source:
Produced in BALB/c mice using highly pure recombinant Human MIP-3beta as the immunizing antigen. This IgG1k antibody was purified from cell culture by Protein A affinity chromatography.
Western Blot:
To detect Human MIP-3beta by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIP-3beta is 2.0-4.0 ng/lane, under reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect recombinant Human MIP-3beta when used with BioGems biotinylated antigen affinity purified anti-Human MIP-3beta (60-228BT) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml.
Immunohistochemistry:
This antibody stained PBMC. The primary antibody was incubated at 5.0 mg/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Produced in BALB/c mice using highly pure recombinant Human MIP-3beta as the immunizing antigen. This IgG1k antibody was purified from cell culture by Protein A affinity chromatography.
Western Blot:
To detect Human MIP-3beta by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIP-3beta is 2.0-4.0 ng/lane, under reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect recombinant Human MIP-3beta when used with BioGems biotinylated antigen affinity purified anti-Human MIP-3beta (60-228BT) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml.
Immunohistochemistry:
This antibody stained PBMC. The primary antibody was incubated at 5.0 mg/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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