MiTF monoclonal, anti-human, mouse, rat

Description: Anti-MiTF Rabbit Monoclonal Antibody . Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Protein Function:
Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'- CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium..

Other Names: Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32, MITF {ECO:0000303|PubMed:8069297, ECO:0000312|HGNC:HGNC:7105}

Clone Name(s):
Clone: AOED-13

Subcellular Localization: Nucleus.

Tissue Specificity: Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. Isoform Mdel is widely expressed in melanocytes, melanoma cell lines and tissues, but almost undetectable in non-melanoma cell lines..

Gene ID: 4286

Immunogen: A synthesized peptide derived from human MiTF

Calculated MW: 58795

Purification: Affinity-chromatography

Format: Liquid

Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Concentration (mg/ml): Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.