Mitochondria (Marker for Human Cells) (clone SPM198), anti-human

Mitochondria (Marker for Human Cells) (clone SPM198), anti-human

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In stock
SKU
ARP-24-MSM1X-739
Catalog Number: 24-MSM1X-739
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG1, kappa
Decription:
This monoclonal antibody recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.

Synonyms: Not Known

Clone: SPM198

Isotype: Mouse IgG1, kappa

Immunogen: Semi-purified mitochondrial preparation

Reactivity: human. Does not react with mouse and rat. Others-not known.

Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Applications: Immunofluorescence (0.5-1ug/ml)
Western Blotting (0.25-0.5ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Reactivity: HeLa or HepG2 cells. Hepatic carcinoma.

Concentration: 0.2mg/ml

Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
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