Mitofusin 1 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
Mitofusin-1 is a protein that in humans is encoded by the MFN1 gene. It is an 80­90 kDa mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed, and found in the outer mitochondrial membrane. The protein encoded by this gene is a mediator of mitochondrial fusion, and thereby contribute to the dynamic balance between fusion and fission that determines mitochondria morphology. MFN1 is known to form oligomers, either with itself or MFN­2, and to undergo ubiquitination by MARCH5. MFN­1 has two key domains. One is a coiled­coil region that mediates MFN­1: MFN­1/2 binding, and a second is a GTPase domain whose cleavage of GTP is necessary for membrane fusion. Overexpression of MFN1 caused perinuclear mitochondrial clustering.

Description:
Rabbit IgG polyclonal antibody for Mitofusin-1 (MFN1) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
Fzo homolog antibody, Hfzo1 antibody, Hfzo2 antibody, MFN 1 antibody, Mfn1 antibody, MFN1_HUMAN antibody, Mitochondrial transmembrane GTPase Fzo 1 antibody, Mitochondrial transmembrane GTPase FZO 2 antibody, Mitochondrial transmembrane GTPaseFZO1B antibody, Mitofusin 1 antibody, Mitofusin-1 antibody, Mitofusin1 antibody, Putative transmembrane GTPase antibody, Transmembrane GTPase MFN1 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminal of human Mitofusin 1 (23-49aa DQLLEFVTEGSHFVEATYKNPELDRIA), different from the related mouse and rat sequences by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.