Mitotic cells (clone 8B3G), anti-human, zebrafish

Background:
The life cycle of a eukaryotic cell consists of various phases, two of which can morphologically and biochemically be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation and spindle formation are microscopically visible. Secondly, in S-phase the DNA of a cell is repliCated, a process that can be detected using biochemical techniques, such as the BrdU incorpoRation assay. In between the M- and S-phase two gap phases occur: the G1-phase, the gap between mitosis and the start of DNA repliCation, and G2-phase, the gap between completion of DNA repliCation and the onset of mitosis. From G1-phase a cell can leave the cell cycle and enter G0, a ‘quiescent' phase. Regulation of the cell cycle predominantly occurs at three major control points, which govern the transition from G0 to G1, from G1 to S, and from G2 to M-phase.

Clone: 8B3G

Isotype: Mouse IgM

Source: 8B3G is a Mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Mouse immunized with a total cell lysate of the Human bladder carcinoma cell line T24.

Reactivity: human, zebrafish

Specificity: 8B3G strongly stains mitotic cells and can therefore be used in flow cytometric analyses of cell suspensions to detect the mitotic index. Together with a quantitative DNA staining procedure (e.g. propidium iodide) 8B3G clearly distinguishes these M-phase cells from cell at other stages of the cell cycle (see figure). Dynamic information can be obtained by combining BrdU incorpoRation with 8B3G staining, which can distinguish and quantitate the four major fractions of the cell cycle.

Form: Each vial contains 100 ul purified monoclonal antibody in PBS containing 0.09% sodium azide.

Applications: Flow Cytometry, Immunocytochemistry

Storage: Store at 4C, or in small aliquots at -20C.