MLCK (Phospho Tyr471) Rabbit polyclonal antibody, anti-human, mouse, rat

Background:
This gene| a muscle member of the immunoglobulin gene superfamily| encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition| using a separate promoter in an intron in the 3' region| it encodes telokin| a small protein identical in sequence to the C-terminus of myosin light chain kinase| that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been

Research Area: Signal transduction

Synonyms: MLCK , Phospho Tyr471, Myosin light chain kinase, smooth muscle, MLCK, smMLCK, EC 2.7.11.18, Kinase-related protein, KRP, Telokin, Myosin light chain kinase, smooth muscle, deglutamylated form,

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration: 1 mg/ml

Storage:
-20°C for 1 year