Monoclonal Anti-Flag/ DYKDDDDKTag

Background:
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. The peptide sequence of the FLAG-tag is: N-DYKDDDDK-C (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein.

Immunogen:
A synthetic peptideDYKDDDDKconjugated to KLH.Western Blot (1:1000-10000); Immunofluorescence, IF (1:200-1000); Immunoprecipitation, IP (1:100-500);

Product Form: Liquid

Storage Buffer: In PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.

Storage Instruction:
Store at 2-8℃ for 1 month. Or aliquot and store at -20℃ or below for long term. Avoid repeated freeze and thaw cycles.