Monoclonal Anti-HA Tag
€0.00
In stock
SKU
BTL1009
Catalog Number: BTL1009
Size: 100 μg
Isotype: Mouse IgG1
Reactivity: All
Application(s): WB, IP, IF, ELISA
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Size: 100 μg
Isotype: Mouse IgG1
Reactivity: All
Application(s): WB, IP, IF, ELISA
Request Information
Background:
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. The vectors have been engineered so that the GST carrier can be cleaved from fusion proteins by digestion with site-specific proteases such as thrombin or blood coagulation factor Xa, following which, the carrier and any uncleaved fusion protein can be removed by absorption on glutathione-agarose. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Immunogen:
A synthetic peptide YPYDVPDYA conjugated to KLH.
Specificity: Detect over-expressed proteins containing the HA epitope tag.Western Blot (1:5000-10000); Immunoprecipitation, IP (1:100-500); Immunofluorescence, IF (1:1000); ELISA (1:2000);
Product Form: Liquid
Storage Instruction:
Store at -20℃ for 1 year. Or aliquot and store at -80℃ for long term. Avoid repeated freeze and thaw cycles.
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. The vectors have been engineered so that the GST carrier can be cleaved from fusion proteins by digestion with site-specific proteases such as thrombin or blood coagulation factor Xa, following which, the carrier and any uncleaved fusion protein can be removed by absorption on glutathione-agarose. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Immunogen:
A synthetic peptide YPYDVPDYA conjugated to KLH.
Specificity: Detect over-expressed proteins containing the HA epitope tag.Western Blot (1:5000-10000); Immunoprecipitation, IP (1:100-500); Immunofluorescence, IF (1:1000); ELISA (1:2000);
Product Form: Liquid
Storage Instruction:
Store at -20℃ for 1 year. Or aliquot and store at -80℃ for long term. Avoid repeated freeze and thaw cycles.
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