Monoclonal Anti-MBP Tag

Background:
Maltose-Binding Protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It has an approximate molecular mass of 42.5 kilodaltons. MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins. The fusion protein binds to amylose columns while all other proteins flow through. The MBP-protein fusion can be purified by eluting the column with maltose.

Immunogen:
Recombinant MBP protein with full length.Western Blot (1:1000-10000);

Product Form: Liquid

Storage Buffer: In PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.

Storage Instruction:
Store at 2-8℃ for 1 month. Or aliquot and store at -20℃ or below for long term. Avoid repeated freeze and thaw cycles.