Monoclonal Anti-Tag-100 Tag

Background:
In molecular biology, extracellular-signal-regulated kinases (ERKs) or classical MAP kinases are widely expressed protein kinase intracellular signalling molecules that are involved in functions including the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. ERKs are known to activate many transcription factors, such as ELK1, and some downstream protein kinases. Disruption of the ERK pathway is common in cancers, especially Ras, c-Raf and receptors such as HER2. Tag-100 tag is an epitope tag composed of a 12-residue peptide, EETARFQPGYRS, derived from the C-termini of mammalian MAPK/ERK kinases.

Immunogen:
A synthetic peptide EETARFQPGYRSconjugated to KLH.

Specificity: Recognize C-terminal, internal and N-terminal Tag-100 tagged proteins.Western Blot (1:1000-10000);

Product Form: Liquid

Storage Buffer: In PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.

Storage Instruction:
Store at -20℃ for 1 year. Or aliquot and store at -80℃ for long term. Avoid repeated freeze and thaw cycles.