Monocyte Antigen (clone IPO-M6), anti-human

Source:
A BALB/c mouse was immunized with human cell line HL-60.
Fusion partner: P3-X63-Ag8.653.

Specifications:
IPO-M6 reacts with human leukemia cell line HL-60 and immuno-precipitates two proteins with MW of 48 and 52 kDa. IPO-M6 does not stain B cell lines Daudy, PHS, Namalwa, RPMI-1788 and T-cell lines CCRF-HSB2, Jurkat and Molt-4.

Species reactivity:
Positive: human.

Applications:
IPO-M6 can be applied for staining of monocytes and up to 10 % of lymphocytes from peripheral blood of healthy donors. Blast cells of patients with AMMonL (M5 following FAB classification), AMMonL (M4) and hairy cells leukemia are IPO-M6 positive. The antigen, defined by IPO-M6, is particularly expressed on blood cells from patients with infectious mononucleosis and CLL. Histiocytes and macrophages are also positive. Malignant cells from patients with AML (M1 and M2), T-ALL, B ALL are IPO-M6 negative.
Flowcytometry: +
Frozen sections: +
Immunoprecipitation: +

Positive control: Lymphocytes from peripheral blood of healthy donors.

References:
- This antibody has not been published