Monocytes, Lymphocyte subsets (clone IPO-74), anti-human

Source:
A BALB/c mouse was mouse with human cell line HL-60.
Fusion partner: P3-X63-Ag8.653.

Specifications:
IPO-74 reacts with human leukemia cell line HL-60 and shows a band at MW 127 kDa (reduced) both in immunoprecipitation and Western blotting. In peripheral blood only subsets of B cells, T cells, neutrophilic and eosinophilic granulocytes are positive. All monocytes are positive. Cell lines U937 and K562 are positive, while Daudy, Raji, Jurkat and Molt-4 are negative, as are IL2-dependent T celllines/clones.

Species reactivity:
Positive: human.

Applications:
Classification of normal and malignant lymphocytes.Flow cytometry: +
Frozen sections: +
Immunoprecipitation: +
Paraffin sections: +
Western blotting: +

Positive control: HL-60, human PBL, tonsil.

References:
- Fleming MG, et al, J Cutan Pathol. 17(2): 77-81 (1990)