Mouse a1ACT (Alpha-1-Antichymotrypsin) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Antichymotrypsin(a1ACT). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-1-Antichymotrypsin(a1ACT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Antichymotrypsin(a1ACT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Antichymotrypsin(a1ACT) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase, Infection immunity, Neuro science, Pulmonology, Hepatology

Alternative Names:
A1-ACT, SERPINA3, AACT, ACT, GIG24, GIG25, Serpin Peptidase Inhibitor Clade A Member 3(Alpha-1 Antiproteinase, Antitrypsin), Cell growth-inhibiting gene 24/25 protein

Sample Type:
serum, plasma and other biological fluids

Assay Type: Sandwich

Standard: 200 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only