Mouse Ab1-42 (Amyloid Beta Peptide 1-42) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amyloid Beta Peptide 1-42(Ab1-42). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amyloid Beta Peptide 1-42(Ab1-42). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amyloid Beta Peptide 1-42(Ab1-42), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Amyloid Beta Peptide 1-42(Ab1-42) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Neuro science,

Sample Type:
serum, plasma and other biological fluids

Assay Type: Sandwich

Standard: 500 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only