Mouse ALDH9A1 (Aldehyde Dehydrogenase 9 Family, Member A1) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldehyde Dehydrogenase 9 Family, Member A1(ALDH9A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aldehyde Dehydrogenase 9 Family, Member A1(ALDH9A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldehyde Dehydrogenase 9 Family, Member A1(ALDH9A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aldehyde Dehydrogenase 9 Family, Member A1(ALDH9A1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Metabolic pathway

Alternative Names:
ALDH4, ALDH7, ALDH9, E3, TMABADH, 4-Trimethylaminobutyraldehyde Dehydrogenase, Aldehyde dehydrogenase E3 isozyme, Gamma-aminobutyraldehyde dehydrogenase

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids.

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only