Mouse Bcl2L (B-Cell CLL/Lymphoma 2 Like Protein) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell CLL/Lymphoma 2 Like Protein(Bcl2L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell CLL/Lymphoma 2 Like Protein(Bcl2L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell CLL/Lymphoma 2 Like Protein(Bcl2L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Cell CLL/Lymphoma 2 Like Protein(Bcl2L) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Immune molecule

Alternative Names:
BCL-XL/S, BCL2L1, BCLX, Bcl-X, bcl-xL, bcl-xS, Apoptosis regulator Bcl-X

Sample Type:
Tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 500 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only