Mouse bTG (Beta-Thromboglobulin) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-Thromboglobulin(bTG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beta-Thromboglobulin(bTG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-Thromboglobulin(bTG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-Thromboglobulin(bTG) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cytokine, Hematology

Alternative Names:
CXCL7, PPBP, PBP, B-TG1, CTAP-III, CTAP3, CTAPIII, LA-PF4, LDGF, MDGF, NAP2, SCYB7, TC1, TC2, TGB1, THBGB1, Pro-Platelet Basic Protein, Chemokine C-X-C-Motif Ligand 7

Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Type: Sandwich

Standard: 100 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only