Mouse CAMP (Cathelicidin Antimicrobial Peptide) ELISA Kit
€0.00
In stock
SKU
ELK5435
Catalog Number: ELK5435
Reactivity: Mouse
Standard Range: 312.5-20000 pg/mL
Sensitivity: 92.5 pg/mL
Information
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Reactivity: Mouse
Standard Range: 312.5-20000 pg/mL
Sensitivity: 92.5 pg/mL
Information
Request Manual
Questions? Contact us!
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cathelicidin Antimicrobial Peptide(CAMP) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathelicidin Antimicrobial Peptide(CAMP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cathelicidin Antimicrobial Peptide(CAMP) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Infection immunity
Alternative Names:
CAP18, FALL39, HSD26, LL37, 18 kDa Cationic Antimicrobial Protein, Antibacterial protein FALL-39, FALL-39 peptide antibiotic
Assay Type: Competitive Inhibition
Standard: 20000 pg/mL
Assay Length: 2h
Assay Length: For Research Use Only
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cathelicidin Antimicrobial Peptide(CAMP) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathelicidin Antimicrobial Peptide(CAMP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cathelicidin Antimicrobial Peptide(CAMP) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Area: Infection immunity
Alternative Names:
CAP18, FALL39, HSD26, LL37, 18 kDa Cationic Antimicrobial Protein, Antibacterial protein FALL-39, FALL-39 peptide antibiotic
Assay Type: Competitive Inhibition
Standard: 20000 pg/mL
Assay Length: 2h
Assay Length: For Research Use Only
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