Mouse CCR6 (Chemokine C-C-Motif Receptor 6) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Receptor 6(CCR6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-C-Motif Receptor 6(CCR6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-C-Motif Receptor 6(CCR6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-C-Motif Receptor 6(CCR6) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction, CD & Adhesion molecule, Infection immunity,

Alternative Names:
CD196, BN-1, CKR-L3, CKR6, CKRL3, CMKBR6, DCR2, DRY-6, GPR-CY4, GPR29, GPRCY4, STRL22, Chemokine receptor-like 3, LARC receptor, G-protein coupled receptor 29

Sample Type:
Tissue homogenates, cell lysates and other biological fluids.

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only