Mouse CGRP (Calcitonin Gene Related Peptide) ELISA Kit

Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Gene Related Peptide(CGRP) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gene Related Peptide(CGRP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gene Related Peptide(CGRP) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Infection immunity, Endocrinology, Neuro science, Hormone metabolism, Bone metabolism, Dermatology

Alternative Names:
CALCB, CGRP2, CALC2, CGRP-II, Calcitonin Gene Related Peptide 2, Calcitonin 2, Calcitonin Related Polypeptide Beta

Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 1000 pg/mL

Assay Length: 2h

Assay Length: For Research Use Only