Mouse CORT (Corticosterone) ELISA Kit
Catalog Number: ACE-E0125
Size: 96 tests/kit
Detection Range: 3.12—200 ng/mL
Sensitivity: 1.93 ng/mL
Datasheet
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Size: 96 tests/kit
Detection Range: 3.12—200 ng/mL
Sensitivity: 1.93 ng/mL
Datasheet
Request Information
Price on request
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You are enquiring about Mouse CORT (Corticosterone) ELISA Kit
Description:
This ELISA kit can be applied to the in vitro quantitative determination of CORT concentrations in serum and plasma.
Assay Principles: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with CORT. During the reaction, CORT in the sample or standard competes with a fixed amount of CORT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to CORT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CORT in the samples is then determined by comparing the OD of the samples to the standard curve.
Species reactivity:
Mouse
Sample Type: serum, plasma
Storage instruction: -20˚C and 4˚C for 6 months
This ELISA kit can be applied to the in vitro quantitative determination of CORT concentrations in serum and plasma.
Assay Principles: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with CORT. During the reaction, CORT in the sample or standard competes with a fixed amount of CORT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to CORT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CORT in the samples is then determined by comparing the OD of the samples to the standard curve.
Species reactivity:
Mouse
Sample Type: serum, plasma
Storage instruction: -20˚C and 4˚C for 6 months
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