Mouse COX1 (Cytochrome C Oxidase Subunit I) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome C Oxidase Subunit I(COX1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cytochrome C Oxidase Subunit I(COX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome C Oxidase Subunit I(COX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome C Oxidase Subunit I(COX1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase

Alternative Names:
MT-CO1, MTCO1, COI, COXI, Mitochondrially Encoded Cytochrome C Oxidase I, Cytochrome c oxidase polypeptide I

Sample Type:
Tissue homogenates, cell lysates and other biological fluids.

Assay Type: Sandwich

Standard: 20 ng/mL

Assay Length: 3.5h

Assay Length: For Research Use Only