Mouse CXCR4 (Chemokine C-X-C-Motif Receptor 4) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X-C-Motif Receptor 4(CXCR4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-X-C-Motif Receptor 4(CXCR4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-X-C-Motif Receptor 4(CXCR4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-X-C-Motif Receptor 4(CXCR4) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction, CD & Adhesion molecule, Tumor immunity, Infection immunity,

Alternative Names:
CD184, Fusin, NPYR, HSY3RR, LAP3, LCR1, LESTR, NPY3R, NPYRL, WHIM, Leukocyte-derived seven transmembrane domain receptor, Lipopolysaccharide-associated protein 3

Sample Type:
tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 5000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only