Mouse CYP27B1 (Cytochrome P450 27B1) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 27B1(CYP27B1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 27B1(CYP27B1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 27B1(CYP27B1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 27B1(CYP27B1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Metabolic pathway

Alternative Names:
VDD1, PDDR, CYP1, P450c1, 25-Hydroxyvitamin D3 1-Alpha-Hydroxylase, Cytochrome P450 Family 27 Subfamily B Polypeptide 1, Calcidiol 1-monooxygenase, VD3 1A hydroxylase

Sample Type:
Tissue homogenates, cell lysates and other biological fluids.

Assay Type: Sandwich

Standard: 40 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only