Mouse D2D (D-Dimer) ELISA Kit

Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with D-Dimer(D2D) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to D-Dimer(D2D). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of D-Dimer(D2D) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Hematology, Cardiovascular biology

Alternative Names:
D 2 Dimer

Sample Type:
plasma

Assay Type: Competitive Inhibition

Standard: 500 ng/mL

Assay Length: 2h

Assay Length: For Research Use Only