Mouse FIL1d (Interleukin 1 Delta) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Delta(FIL1d). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Delta(FIL1d). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Delta(FIL1d), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Delta(FIL1d) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cytokine, Tumor immunity, Infection immunity

Alternative Names:
IL1HY1, IL36RN, IL36Ra, IL1RP3, IL1L1, IL1F5, Interleukin 1 Family, Member 5, Interleukin-1 HY1, IL-1 Related Protein 3, Interleukin 36 Receptor Antagonist

Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 100 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only