Mouse Flt3L (FMS Like Tyrosine Kinase 3 Ligand) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FMS Like Tyrosine Kinase 3 Ligand(Flt3L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FMS Like Tyrosine Kinase 3 Ligand(Flt3L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FMS Like Tyrosine Kinase 3 Ligand(Flt3L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FMS Like Tyrosine Kinase 3 Ligand(Flt3L) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cytokine, Immune molecule, Hematology

Alternative Names:
FLT3LG, FLT3-LG, Flt3-L, FL, Fms-Related Tyrosine Kinase 3 ligand, SL cytokine

Sample Type:
Serum, plasma and other biological fluids.

Assay Type: Sandwich

Standard: 50 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only