Mouse IgG3 (Immunoglobulin G3) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin G3(IgG3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Immunoglobulin G3(IgG3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin G3(IgG3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin G3(IgG3) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Infection immunity, Immune molecule

Alternative Names:
IGHG3, HDC, Heavy chain disease protein, Ig Gamma-3 Chain C Region, Immunoglobulin Heavy Constant Gamma 3, G3m Marker, Immunoglobulin Gm3

Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 200 ug/mL

Assay Length: 4.5h

Assay Length: For Research Use Only