Mouse IL-33 recombinant active protein
€450.00
In stock
SKU
BT-CG73
Background Information: Mouse Interleukin 33 (IL-33) is a 30 kDa proinflammatory cytokine which may also regulates gene transcription in producer cells. IL-33 is constitutively expressed in smooth muscle and airway epithelia. IL-33 was identified based on sequence and structural homology with IL-1 family cytokines. It is up-regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1. BindingIL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces the expression of IL-4, IL-5, IL-13 and also leads to severe pathological changes in mucosal organs.
Target Name:
Interleukin 33; IL-33; IL33; C9orf26; NKHEV; Interleukin-1 family member 11; DVS27; NF-HEV and IL- 1F11
Source: Recombinant Mouse IL-33 (accession number Q8BVZ5) (Ser109-Ile266) is expressed in E.coli system.
Molecular Weight:
The 158 amino acid protein has the predicted molecular weight of 17.6 kDa. The protein migrates at approximately 18kDa on SDS-PAGE with DTT-reduced condition.
Bioactivity:
Immobilized Mouse IL-33 (Cat#CG73) at 10μg/ml (100 μl/well) can bind Mouse ST2-Fc (Cat#CK95). The ED50 of Mouse IL-33 (Cat#CG73) is 1-5 ug/ml .
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, 1mMDTT,pH7.4.
Endotoxin level: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Reconstitution:
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage and Handling:
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Target Name:
Interleukin 33; IL-33; IL33; C9orf26; NKHEV; Interleukin-1 family member 11; DVS27; NF-HEV and IL- 1F11
Source: Recombinant Mouse IL-33 (accession number Q8BVZ5) (Ser109-Ile266) is expressed in E.coli system.
Molecular Weight:
The 158 amino acid protein has the predicted molecular weight of 17.6 kDa. The protein migrates at approximately 18kDa on SDS-PAGE with DTT-reduced condition.
Bioactivity:
Immobilized Mouse IL-33 (Cat#CG73) at 10μg/ml (100 μl/well) can bind Mouse ST2-Fc (Cat#CK95). The ED50 of Mouse IL-33 (Cat#CG73) is 1-5 ug/ml .
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, 1mMDTT,pH7.4.
Endotoxin level: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Reconstitution:
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage and Handling:
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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