Mouse MIP4a (Macrophage Inflammatory Protein 4 Alpha) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 4 Alpha(MIP4a). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 4 Alpha(MIP4a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 4 Alpha(MIP4a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 4 Alpha(MIP4a) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cytokine, Infection immunity, Dermatology

Alternative Names:
CCL26, SCYA26, IMAC, TSC-1, Eotaxin 3, Thymic stroma chemokine-1, Chemokine(C-C-Motif)ligand 26, Thymic Stroma Chemokine-1, Small Inducible Cytokine Subfamily A 26

Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 1000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only