Mouse MRC2 (Mannose Receptor C Type 2) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose Receptor C Type 2(MRC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannose Receptor C Type 2(MRC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose Receptor C Type 2(MRC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannose Receptor C Type 2(MRC2) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Infection immunity, Developmental science

Alternative Names:
CD280, ENDO180, CLEC13E, UPARAP, C-type lectin domain family 13 member E, Endocytic receptor 180, Macrophage mannose receptor 2, Urokinase receptor-associated protein

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids.

Assay Type: Sandwich

Standard: 1000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only