Mouse PDZK1IP1 (PDZK1-interacting protein 1) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDZK1IP1(PDZK1-interacting protein 1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PDZK1IP1(PDZK1-interacting protein 1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDZK1IP1(PDZK1-interacting protein 1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PDZK1IP1(PDZK1-interacting protein 1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cancer

Alternative Names:
MAP17, 17 kDa membrane associated protein, DD96, Epithelial protein up regulated in carcinoma membrane associated protein 17, Membrane associated protein 17, PDZK1-interacting protein 1, Pdzk1ip1, RP1-18D14.5, SPAP,

Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 2000 pg/mL

Assay Length: 3.5h

Assay Length: For Research Use Only