Mouse Pro-MMP-9 (Pro-Matrix Metalloproteinase 9) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pro-MMP-9(Pro-Matrix Metalloproteinase 9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pro-MMP-9(Pro-Matrix Metalloproteinase 9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pro-MMP-9(Pro-Matrix Metalloproteinase 9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pro-MMP-9(Pro-Matrix Metalloproteinase 9) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase, Tumor immunity, Infection immunity, Cardiovascular biology, Hepatology

Alternative Names:
Pro-MMP-9

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids.

Assay Type: Sandwich

Standard: 50 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only