Mouse S17aH (17-Alpha-Hydroxylase) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 17-Alpha-Hydroxylase(S17aH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 17-Alpha-Hydroxylase(S17aH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 17-Alpha-Hydroxylase(S17aH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 17-Alpha-Hydroxylase(S17aH) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase, Metabolic pathway, Endocrinology, Cardiovascular biology, Hepatology, Gastroenterology, Nutrition metabolism

Alternative Names:
CYP17A1, CPT7, P450C17, 17-alpha-hydroxyprogesterone aldolase/17,20-Lyase, Steroid 17-alpha-monooxygenase, Cytochrome P450,Family 17,Subfamily A,Polypeptide 1

Sample Type:
Tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 100 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only