Mouse SHBG (Sex Hormone Binding Globulin) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sex Hormone Binding Globulin(SHBG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sex Hormone Binding Globulin(SHBG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sex Hormone Binding Globulin(SHBG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sex Hormone Binding Globulin(SHBG) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Endocrinology, Reproductive science

Alternative Names:
ABP, TEBG, SBP, Sex steroid-binding protein, Testis-specific androgen-binding protein, Testosterone-estradiol-binding globulin, Testosterone-estrogen-binding globulin

Sample Type:
serum, plasma and other biological fluids

Assay Type: Sandwich

Standard: 2000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only