Mouse Spleen Lysate
€175.00
In stock
SKU
ECM-SL7061
Background:
Whole spleen was dissected from adult mouse (Balb/c). The tissue was rinsed in Hank’s buffered saline solution and lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 10 mM Tris (pH 7.4) buffer. Spleen lysates were sonicated then diluted to the final concentration in electrophoresis sample buffer.
Application dilution:
WB: 20 μl/lane
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Buffer/Storage:
Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.
Whole spleen was dissected from adult mouse (Balb/c). The tissue was rinsed in Hank’s buffered saline solution and lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 10 mM Tris (pH 7.4) buffer. Spleen lysates were sonicated then diluted to the final concentration in electrophoresis sample buffer.
Application dilution:
WB: 20 μl/lane
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Buffer/Storage:
Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.
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