Mouse TAF12 (TATA Box Binding Protein Associated Factor 12) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TATA Box Binding Protein Associated Factor 12(TAF12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TATA Box Binding Protein Associated Factor 12(TAF12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TATA Box Binding Protein Associated Factor 12(TAF12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TATA Box Binding Protein Associated Factor 12(TAF12) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction,

Alternative Names:
TAF2J, TAFII20, Transcription Initiation Factor TFIID Subunit 12, Transcription initiation factor TFIID 20/15 kDa subunits

Sample Type:
Tissue homogenates and other biological fluids.

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only